Wound Molecular Testing Kit
WOUND KIT
The wound kit is a comprehensive kit detecting, by Molecular Technique, the most common pathogens infected wounds and soft skin. Our kit can quickly manage skin and soft tissue infections, avoiding delays in diagnosis and preventing infections from progressing.
ACCURACY AND QUICK RESULTS
Now your lab can provide healthcare providers with the quickest and most accurate results possible to allow them to determine an accurate diagnosis:
- Expedited patient results
- Extensive wound pathogens kit
- PLUS Resistance Markers to avoid ineffective antibiotics
- Simple Collection
- Unmatched accuracy of PCR
- Direct access to experience scientific staff
- Personal customer service experience
For Research Use Only. Not for use In diagnostic procedures.
Pathogens Detected in the DTPM Wound Molecular Testing Kit:
Strain(s) Identified* | Target Gene | GenBank Accession Number | Amplicon1 (base pairs) | BLAST Score2 Range (High/Low) | Sequences3 Reviewed | Matches4 (True Positives) | Specificity5 |
---|---|---|---|---|---|---|---|
Acinetobacter baumannii, A. seifertii, A. nosocomialis, A. gerneri | RecA | NZ_CP018664 | 72 | 128/98 | 1.02E+08 | 778 | 100% |
Bacteroides fragilis, B. caccae, B. dorei, B. eggerthii, B. faecis, B. finegoldii, B. graminisolvens, B. heparinolyticus, B. intestinalis, B. kribbi, B. koreensis, B. luhongzhouii, B. massiliensis, B. ovatus, B. pyogenes, B. stercoris, B. salyersiae, B. thetaiotaomicron, B. uniformis, B. vulgatus, B. xylanisolvens, B. zhangwenhongii, Phocaeicola massiliensis, P. plebeius, P vulgatus | 16S Ribosomal | AP006841.1 | 112 | 187/179 | 1.02E+08 | 29143 | 100% |
Citrobacter freundii, C braakii, C arsenatis, C cronae, C europaeus, C pasteurii, C portucalensis, C rodentium, C werkmanii, C youngae [Salmonella entericaay > 100,000 copies may give weak signal] | gyrA | NZ_CP007557 | 64 | 128/123 | 1.02E+08 | 847 | 100% |
Enterobacter cloacae, E. hormaechei, E. roggenkampii, E. asburiae, E. ludwigii, E. kobei, E. bugandensis | apbC | NZ_CP009756 | 72 | 138/133 | 1.02E+08 | 748 | 100% |
E faecalis | recG | NZ_CP091198 | 66 | 132/122 | 1.02E+08 | 619 | 100% |
Enterococcus faecium | gyrA | NZ_CP038996.1 | 64 | 128/111 | 1.02E+08 | 618 | 100% |
Escherichia coli, O55H7, O157:H7, O10:H32, O104:H4, O121:H19, O178:H19, O6:H16, O169:H41, O25:H16, O27:H7, O128:H27, O114:H49, O15:H11, O6:H16, O127:H6, O4:H5, 025:H1, O8:H28, O170:H18, 085:H1, 0158:H24. Shigellla boydii, S. flexneri, S. dysenteriae | lacY | NC_000913 | 75 | 147/137 | 1.02E+08 | 4629 | 100% |
Klebsiella aerogenes, Enterobacter aerogenes, Raoultella ornithinolytica, R planticola | Omp35 | NC_015663 | 59 | 118/108 | 1.02E+08 | 142 | 100% |
Klebsiella oxytoca, K grimontii, K michiganensis, Raoultella terrigena | TonB | NZ_CP033844 | 60 | 117/112 | 1.02E+08 | 179 | 100% |
Klebsiella variicola, Klebsiella quasipneumoniae, Klebsiella africana | gyrA | NC_016845 | 57 | 114/105 | 1.02E+08 | 3138 | 100% |
Klebsiella pneumoniae, K variicola, K quasipneumoniae, K africana | gyrA | NC_016845 | 57 | 114/107 | 1.02E+08 | 3189 | 100% |
Morganella moranii | gyrA | CP014026 | 65 | 130/120 | 1.02E+08 | 63 | 100% |
Proteus mirabilis | gyrA | NC_010554.1 | 61 | 122 | 1.02E+08 | 143 | 100% |
Proteus vulgaris, P columbae, P hauseri, P penneri, P terrae [P mirabilis > 50,0000 copies may give a weak signal] | gyrA | NZ_CP090064.1 | 64 | 128 | 1.02E+08 | 48 | 100% |
Psuedomonas aeruginosa, P. alcaligenes, P. balearica, P. citronellolis, P. furukawaii, P. fluorescens, P. guangdongensis, P. jinjuensis, P. knackmussii, P. lalkuanensis, P. multiresinivorans, P. nitroreducens, P. otitidis, P. oryzihabitans, P. stutzeri, P. tohonis, P. paraeruginosa, Stutzerimonas balearica, S. frequens, S. stutzeri | oprL | NC_002516.2 | 60 | 120/90 | 1.02E+08 | 2035 | 100% |
Staphylococcus aureus | acetyl-CoA acetyltransferase | NC_007795 | 59 | 112/107 | 1.02E+08 | 1912 | 100% |
Staphylococcus aureus | acetyl-CoA-acetyltransferase | NC_007795 | 59 | 112/104 | 1.02E+08 | 1969 | 100% |
Streptococcus pyogenes | gyrA | CP014278.2 | 62 | 124/119 | 1.02E+08 | 292 | 100% |
Footnotes:
- The Amplicon is the amplified piece of DNA which contains the Fwd, Rev and Probe sequence. Sequence between the Fwd, Rev and Probe are also included in Amplicon.
- The BLAST Score describes how similar the Amplicon sequence is to aligned sequences in the non-redundant GenBank Database. The range shown as High/Low reflects the highest and lowest scores for a pathogen that would generate a positive response using this assay.
- The number of sequences reviewed reflect the total number sequences in the non-redundant GenBank database on the date of the analysis. This number will change as new sequences are added to the GenBank database.
- Matches reflect the total number of pathogens identified in the BLAST search that would generate a positive ressponse. Statistically these are the number of True Positives (the diagnostic test would indicates the presence of the correct disease) = TP
- False positive (FP) – Test incorrectly detects the presence of pathogen in a sample that does not contain the pathogen. Shown as an incorrect pathogen in the BLAST analysis with a Score within the High/Low range listed for the assay.
True negative (TN) – Test correctly identifies absence of pathogen. Calculated from BLAST data as the (total number of sequences reviewed) – (total number of pathogens correctly detected or TP).
Specificity = TN / TN + FP
https://lexjansen.com/nesug/nesug10/hl/hl07.pdf
* If you have inquiries about specific strains, please contact DTPM support.