Wound Molecular Testing Kit

WOUND KIT

The wound kit is a comprehensive kit detecting, by Molecular Technique, the most common pathogens infected wounds and soft skin. Our kit can quickly manage skin and soft tissue infections, avoiding delays in diagnosis and preventing infections from progressing.

ACCURACY AND QUICK RESULTS

Now your lab can provide healthcare providers with the quickest and most accurate results possible to allow them to determine an accurate diagnosis:

  • Expedited patient results
  • Extensive wound pathogens kit
  • PLUS Resistance Markers to avoid ineffective antibiotics
  • Simple Collection
  • Unmatched accuracy of PCR
  • Direct access to experience scientific staff
  • Personal customer service experience

For Research Use Only. Not for use In diagnostic procedures.

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Pathogens Detected in the DTPM Wound Molecular Testing Kit:

Strain(s) Identified* Target Gene GenBank Accession Number Amplicon1 (base pairs) BLAST Score2 Range (High/Low) Sequences3 Reviewed Matches4 (True Positives) Specificity5
Acinetobacter baumannii, A. seifertii, A. nosocomialis, A. gerneri RecA NZ_CP018664 72 128/98 1.02E+08 778 100%
Bacteroides fragilis, B. caccae, B. dorei, B. eggerthii, B. faecis, B. finegoldii, B. graminisolvens, B. heparinolyticus, B. intestinalis, B. kribbi, B. koreensis, B. luhongzhouii, B. massiliensis, B. ovatus, B. pyogenes, B. stercoris, B. salyersiae, B. thetaiotaomicron, B. uniformis, B. vulgatus, B. xylanisolvens, B. zhangwenhongii, Phocaeicola massiliensis, P. plebeius, P vulgatus 16S Ribosomal AP006841.1 112 187/179 1.02E+08 29143 100%
Citrobacter freundii, C braakii, C arsenatis, C cronae, C europaeus, C pasteurii, C portucalensis, C rodentium, C werkmanii, C youngae [Salmonella entericaay > 100,000 copies may give weak signal] gyrA NZ_CP007557 64 128/123 1.02E+08 847 100%
Enterobacter cloacae, E. hormaechei, E. roggenkampii, E. asburiae, E. ludwigii, E. kobei, E. bugandensis apbC NZ_CP009756 72 138/133 1.02E+08 748 100%
E faecalis recG NZ_CP091198 66 132/122 1.02E+08 619 100%
Enterococcus faecium gyrA NZ_CP038996.1 64 128/111 1.02E+08 618 100%
Escherichia coli, O55H7, O157:H7, O10:H32, O104:H4, O121:H19, O178:H19, O6:H16, O169:H41, O25:H16, O27:H7, O128:H27, O114:H49, O15:H11, O6:H16, O127:H6, O4:H5, 025:H1, O8:H28, O170:H18, 085:H1, 0158:H24. Shigellla boydii, S. flexneri, S. dysenteriae lacY NC_000913 75 147/137 1.02E+08 4629 100%
Klebsiella aerogenes, Enterobacter aerogenes, Raoultella ornithinolytica, R planticola Omp35 NC_015663 59 118/108 1.02E+08 142 100%
Klebsiella oxytoca, K grimontii, K michiganensis, Raoultella terrigena TonB NZ_CP033844 60 117/112 1.02E+08 179 100%
Klebsiella variicola, Klebsiella quasipneumoniae, Klebsiella africana gyrA NC_016845 57 114/105 1.02E+08 3138 100%
Klebsiella pneumoniae, K variicola, K quasipneumoniae, K africana gyrA NC_016845 57 114/107 1.02E+08 3189 100%
Morganella moranii gyrA CP014026 65 130/120 1.02E+08 63 100%
Proteus mirabilis gyrA NC_010554.1 61 122 1.02E+08 143 100%
Proteus vulgaris, P columbae, P hauseri, P penneri, P terrae [P mirabilis > 50,0000 copies may give a weak signal] gyrA NZ_CP090064.1 64 128 1.02E+08 48 100%
Psuedomonas aeruginosa, P. alcaligenes, P. balearica, P. citronellolis, P. furukawaii, P. fluorescens, P. guangdongensis, P. jinjuensis, P. knackmussii, P. lalkuanensis, P. multiresinivorans, P. nitroreducens, P. otitidis, P. oryzihabitans, P. stutzeri, P. tohonis, P. paraeruginosa, Stutzerimonas balearica, S. frequens, S. stutzeri oprL NC_002516.2 60 120/90 1.02E+08 2035 100%
Staphylococcus aureus acetyl-CoA acetyltransferase NC_007795 59 112/107 1.02E+08 1912 100%
Staphylococcus aureus acetyl-CoA-acetyltransferase NC_007795 59 112/104 1.02E+08 1969 100%
Streptococcus pyogenes gyrA CP014278.2 62 124/119 1.02E+08 292 100%
Footnotes:

  1. The Amplicon is the amplified piece of DNA which contains the Fwd, Rev and Probe sequence. Sequence between the Fwd, Rev and Probe are also included in Amplicon.
  2. The BLAST Score describes how similar the Amplicon sequence is to aligned sequences in the non-redundant GenBank Database. The range shown as High/Low reflects the highest and lowest scores for a pathogen that would generate a positive response using this assay.
  3. The number of sequences reviewed reflect the total number sequences in the non-redundant GenBank database on the date of the analysis. This number will change as new sequences are added to the GenBank database.
  4. Matches reflect the total number of pathogens identified in the BLAST search that would generate a positive ressponse. Statistically these are the number of True Positives (the diagnostic test would indicates the presence of the correct disease) = TP
  5. False positive (FP) – Test incorrectly detects the presence of pathogen in a sample that does not contain the pathogen. Shown as an incorrect pathogen in the BLAST analysis with a Score within the High/Low range listed for the assay.
    True negative (TN) – Test correctly identifies absence of pathogen. Calculated from BLAST data as the (total number of sequences reviewed) – (total number of pathogens correctly detected or TP).

Specificity = TN / TN + FP
https://lexjansen.com/nesug/nesug10/hl/hl07.pdf

* If you have inquiries about specific strains, please contact DTPM support.

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