Respiratory Molecular Testing Kit RPP
RESPIRATORY KIT
Our Respiratory Kit provides comprehensive detection by PCR of viral and bacterial pathogens associated with respiratory illness.
- Acute Bronchitis
- Common Cold
- Acute Pharyngitis
- Acute Tonsillitis
- Cough and Fever
- Wheezing
- Pneumonia
- Viral Infection
- Other Nasal and Sinus Diseases
ACCURACY AND QUICK RESULTS
Now your lab can provide healthcare providers with the quickest and most accurate results possible to allow them to determine an accurate diagnosis:
- Expedited patient results
- Multiple pathogens available
- Unmatched accuracy of PCR
- Simple collection
- Direct access to experienced scientific staff
- Personal customer service experience
For Research Use Only. Not for use In diagnostic procedures.
Pathogens Detected in the DTPM Respiratory Molecular Testing Kit:
Pathogen | Strain(s) Identified* | Target Gene | GenBank Accession Number | Amplicon1 (base pairs) | BLAST Score2 Range (High/Low) | Sequences3 Reviewed | Matches4 (True Positives) | Specificity5 |
---|---|---|---|---|---|---|---|---|
Adenovirus (RPP) | Adenovirus 3, 4, 4a, 4p. 7, 10, 11, 13, 14, 16, 17, 21, 21a, 34, 35, 40, 48, 50, 51, 55, 71, B3, E4, E4, Human mastadenovirus B, D, E | L3 | NC_011203 | 59 | 100/90 | 1.02E+08 | 1637 | 100% |
Bocavirus | Human Bocavirus 1, Primate bocaparvovirus 1 | VP2 | KU557406 | 62 | 124/114 | 1.02E+08 | 643 | 100% |
Bordetella pertussis V 3.0 | Bordetella pertussis | ptxS1 | CP046994 | 79 | 155/150 | 1.02E+08 | 991 | 100% |
Bordetella pertussis/holmesii | Bordetella pertussis, holmesii (Bordetella bronchiseptica) | Integrase | CP046994 | 54 | 108/103 | 1.02E+08 | 1059 | 100% |
Chlamydia pneumoniae [previously Chlamydophila pneumoniae] | Chlamydia pneumoniae [previously known as Chlamydophila pneumoniae] | porin | NC_005043 | 66 | 132/112 | 1.02E+08 | 33 | 100% |
Coronavirus 229E | Human coronavirus 229, Bat coronavirus | N | NC_002645 | 74 | 148/132 | 1.02E+08 | 241 | 100% |
Coronavirus HKU-1 | Human coronavirus HKU-1 | N | NC_006577 | 78 | 147/142 | 1.02E+08 | 143 | 100% |
Coronavirus NL63 | Human coronavirus NL-63 | N | NC_005831 | 67 | 134/129 | 1.02E+08 | 226 | 100% |
Coronavirus OC43 | Human coronavirus OC43, Bovine, Dromedary camel , Canine, Yak, Deer (Water, White-tailed ), Waterbuck, Giraffe, Sable antelope, Porcine, Rabbit | N | NC_006213 | 65 | 130/108 | 1.02E+08 | 955 | 100% |
Entertovirus / Rhinovirus (EV/RV)* | Rhino A, B, C: Enterovirus D68, A23, A47, A71, Coxsackievirus A1, A6, A11, A13, A16, A20, A22, Echovirus, Human poliovirus 1, 2, 3, *Pathogens associated with hand, foot, and mouth disease (Coxsackievirus A16, A6, Enterovirus 71) are detected by this assay | Polyprotein | NC_038308 & NC_038311 | 114 | 171/98 | 1.02E+08 | 7798 | 100% |
Haemophilus influenzae | Haemophilus aegyptius | Protein D (hpd) | CP029621 | 67 | 125/120 | 1.02E+08 | 142 | 100% |
Influenza A | H3N2, H1N1, H5N1, H3N2, H1(2009), H7N9 [inquire about other strains] | M1/M2 | NC_007367 | 98 | 161/129 | 1.02E+08 | 54079 | 100% |
Influenza B | B/Victoria, B/Yamagata | NEP | NC_002211 | 66 | 126/116 | 1.02E+08 | 7064 | 100% |
Klebsiella pneumoniae | Klebsiella variicola, Klebsiella quasipneumoniae, Klebsiella africana | gyrA | NC_016845 | 57 | 114/105 | 1.02E+08 | 3138 | 100% |
Klebsiella pneumoniae | Klebsiella pneumoniae, K variicola, K quasipneumoniae, K africana | gyrA | NC_016845 | 57 | 114/107 | 1.02E+08 | 3189 | 100% |
Legionella pneumophila | Legionella worsleiensis & Legionella fairfieldensis | mip | NZ_CP015941 | 79 | 155/143 | 1.02E+08 | 366 | 100% |
Metapneumovirus A & B | A2, B2 | F | NC_039199 | 79 | 143/100 | 1.02E+08 | 1624 | 100% |
Moraxella catarrhalis | Moraxella catarrhalis | UspA1 | CP018059 | 65 | 130 | 1.02E+08 | 31 | 100% |
Mycoplasma pneumoniae | Mycoplasma pneumoniae | P1 | CP039772 | 61 | 122/111 | 1.02E+08 | 129 | 100% |
Parainfluenza virus Type 1 | HPV 1, Human respirovirus 1 | L RNA polymerase | NC_003461 | 74 | 142/132 | 1.02E+08 | 114 | 100% |
Parainfluenza virus Type 2 | Human Rubelavirus 2, Human orthorubelavirus 2 | NP | NC_003443 | 68 | 124 | 1.02E+08 | 83 | 100% |
Parainfluenza virus Type 3 | HPV 3, Human respirovirus 3 | N | NC_001796 | 75 | 141/131 | 1.02E+08 | 580 | 100% |
Parainfluenza virus Type 4 | HPV 4, Human parainfluenza virus 4a & 4b, Human orthorubulavirus 4 | HN | NC_021928 | 118 | 188/141 | 1.02E+08 | 157 | 100% |
Parechovirus | A, 1,3,4,5,6,8,17,19 | 5′ UTR Polyprotein | NC_001897 | 146 | 146/114 | 1.02E+08 | 460 | 100% |
Respiratory Syncytial Virus A & B | A,B, Human orthopneumovirus | L | NC_001781 | 125 | 214/199 | 1.02E+08 | 5343 | 100% |
Staphylococcus aureus | Staphylococcus aureus | acetyl-CoA acetyltransferase | NC_007795 | 59 | 112/107 | 1.02E+08 | 1912 | 100% |
Staphylococcus aureus | Staphylococcus aureus | acetyl-CoA-acetyltransferase | NC_007795 | 59 | 112/104 | 1.02E+08 | 1969 | 100% |
Streptococcus dysgalactiae | Streptococcus dysgalactiae | gyrA | AP012976.1 | 56 | 112/103 | 1.02E+08 | 62 | 100% |
Streptococcus pneumoniae | Streptococcus pneumoniae | lytA | CP003357 | 60 | 120/110 | 1.02E+08 | 375 | 100% |
Footnotes:
- The Amplicon is the amplified piece of DNA which contains the Fwd, Rev and Probe sequence. Sequence between the Fwd, Rev and Probe are also included in Amplicon.
- The BLAST Score describes how similar the Amplicon sequence is to aligned sequences in the non-redundant GenBank Database. The range shown as High/Low reflects the highest and lowest scores for a pathogen that would generate a positive response using this assay.
- The number of sequences reviewed reflect the total number sequences in the non-redundant GenBank database on the date of the analysis. This number will change as new sequences are added to the GenBank database.
- Matches reflect the total number of pathogens identified in the BLAST search that would generate a positive ressponse. Statistically these are the number of True Positives (the diagnostic test would indicates the presence of the correct disease) = TP
- False positive (FP) – Test incorrectly detects the presence of pathogen in a sample that does not contain the pathogen. Shown as an incorrect pathogen in the BLAST analysis with a Score within the High/Low range listed for the assay.
True negative (TN) – Test correctly identifies absence of pathogen. Calculated from BLAST data as the (total number of sequences reviewed) – (total number of pathogens correctly detected or TP).
Specificity = TN / TN + FP
https://lexjansen.com/nesug/nesug10/hl/hl07.pdf
* If you have inquiries about specific strains, please contact DTPM support.