Nail Fungal
Molecular Testing Kit
NAIL FUNGAL KIT
The DTPM Nail Fungal kit is specifically designed to test for pathogens in the aid of fast identification of nail fungal infections. Toenail or fingernail samples can be collected with no debridement of the nailbed needed.
SYMPTOMS TO CONSIDER:
Those patients affected may experience some of the following symptoms:
- Thickened nails
- Whiteish to yellow-brown discoloration
- Brittle, crumbly, or ragged nail
- A dark coloration or distorted shape
- A new or odd odor
COLLECTION METHOD
The DTPM Nail Fungal kit utilizes a 3mm – 6mm (or larger) nail specimen collected by a healthcare professional.
ACCURACY AND QUICK RESULTS
The DTPM Nail Fungal Kit will allow your laboratory to provide healthcare professionals with the fastest and most accurate detection of common nail fungal organisms.
- Expedited patient results
- Multiple pathogens available
- Unmatched accuracy of PCR
- Simple collection
- Direct access to experienced scientific staff
- Personal customer service experience
For Research Use Only. Not for use In diagnostic procedures.
PATHOGENS DETECTED IN THE DTPM NAIL FUNGAL MOLECULAR TESTING KIT:
Pathogen | Strain(s) Identified* | Target Gene | GenBank Accession Number | Amplicon1 (base pairs) | BLAST Score2 Range (High/Low) | Sequences3 Reviewed | Matches4 (True Positives) | Specificity5 |
---|---|---|---|---|---|---|---|---|
Acremonium (Sarocladium) strictum | Acremonium (Sarocladium) strictum, S bactrocephalum, Sordariomycetes, Hypocreales, Lichtheimia corymbifera, Colletotrichum, Diaporthe eres, Valsa ambiens | ITS Ribosomal | NR_111145.1 | 121 | 239/200 | 1.02E+08 | 599 | 100% |
Acremonium potronii | Acremonium potronii, A dichromosporum, A fuci, A persicinum, A salmoneum, A sclerotigenum, A zonatum, Emericellopsis maritima, E alkalina, E cladophorae, E phycophila, E pallida, E stolkiae, E nidulans, E atlantica, Mycocitrus zonatus, Hypocreales, Parasarocladium breve, Stilbella fimetaria | ITS Ribosomal | JX535048.1 | 56 | 100/95 | 1.02E+08 | 219 | 100% |
Alternaria species | Alternaria species, Embellisia dennisii, E conoidea, E indefessa, Ulocladium dauci, U capsicum, U oudemansii, U obovoideum, septosporum, Nimbya perpunctulata, N perpunctulata, N celosiae | Alta1 | CM034651 | 84 | 147/110 | 1.02E+08 | 2441 | 100% |
Aspergillus flavus / oryzae v2.0 | Aspergillus flavus, A oryzae {*aflatoxin biosynthesis gene] | aflC | NC_054693.1 | 67 | 125/120 | 1.02E+08 | 47 | 100% |
Aspergillus fumigatus | Aspergillus fumigatus, A aculeatus, A arcoverdensis, A aureolus, A brevipes, A botucatensis, A chermesinum, A delicatus, A duricaulis, A felis, A fischeri, A frankstonensis, A fumigatiaffinis, A fumisynnematus, A hiratsukae, A igneus, A laciniosus, A lentulus, A malignus, A marvanovae, A nishimurae, A novofumigatus, A oerlinghausenensis, A parafelis, A pseudofelis, A pseudoviridinutans, A shendaweii, A siamensis, A spinosus, A takakii, A terrus, A tsunodae, A turcosus, A udagawae, A versicolor, A viridinutans, A waksmanii, A wyomingensis, Neosartorya aureola, N coreana, N fischeri, N hiratsukae, N laciniosa, N pseudofischeri, N spathulata, N spinosa, N udagawae, Penicillium chermesinum, P glandicola | ITS Ribosomal | NC_007197 | 101 | 193/181 | 1.02E+08 | 4220 | 100% |
Aspergillus niger / tubingensis | Aspergillus niger, A tubingensis, A aculeatus, A amstelodami, A awamori, A brasiliensis, A costaricensis, A cristatus, A ficuum, A foetidus, A lacticoffeatus, A luchuensis, A neoniger, A phoenicis, A piperis, A sclerotiorum, A terreus, A welwitschiae, Actinomucor elegans | ITS Ribosomal | CM033103 | 72 | 144/131 | 1.02E+08 | 5186 | 100% |
Aspergillus terreus | Aspergillus terreus, A alabamensis, A aureoterreus, A citrinoterreus, A elongatus A hortai, A jilinensis, A montoensis, A neoafricanus, A nomius, A pseudoterreus, A temulenta | ITS Ribosomal | LC496504 | 58 | 109/95 | 1.02E+08 | 1318 | 100% |
Candida albicans | Candida albicans, Candid dubliniensis | Erg11 | NC_032093 | 74 | 142/134 | 1.02E+08 | 363 | 100% |
Candida parapsilosis | Candida parapsilosis | Erg11 | NW_023503279 | 65 | 130/130 | 1.02E+08 | 502 | 100% |
Candida tropicalis | Candida tropicalis | Erg11 | NW_003020060 | 70 | 140/134 | 1.02E+08 | 369 | 100% |
Epidermophyton floccosum | Epidermophyton floccosum, Arthroderma uncinatum | ITS Ribosomal | MH858667.1 | 63 | 126/121 | 1.02E+08 | 166 | 100% |
Fusarium solani | Fusarium solani, F akasia, F ambrosium, F breve, F ensiforme, F euwallaceae, F falciforme, F ferrugineum, F haematococcum, F keratoplasticum, F lateritium, F lichenicola, F metavorans, F neocosmosporiellum, F oblongum, F oxysporum, F paranaense, F perseae, F petroliphilum, F phaseoli, F populicola, F pseudensiforme, F rekanum, F striatum, F suttonianum, F tonkinense, F vanettenii, F waltergamsii, F witzenhausenense, Earliella scabrosa, Ascomycota, Nectria haematococca, N ipomoeae, Neocosmospora vasinfecta, Sordariomycetes | ITS Ribosomal | KY484966.1 | 164 | 319/241 | 1.02E+08 | 10871 | 100% |
Microsporum spp | Microsporum canis, M ferrugineum, M audouinii, Arthroderma otae | ITS Ribosomal | LC317666 | 59 | 118/100 | 1.02E+08 | 965 | 100% |
Nakaseomyces glabratus [previously Candida glabrata] | Nakaseomyces glabratus previously known as Candida glabrata | Erg11 | NC_006028 | 64 | 128/128 | 1.02E+08 | 101 | 100% |
Neofusicoccum mangiferae | Neofusicoccum mangiferae, N eucalyptorum, N parvum, Botryosphaeria eucalyptorum | ITS Ribosomal | MN519717.1 | 80 | 157/148 | 1.02E+08 | 138 | 100% |
Neoscytalidium (Scytalidium) dimidiatum | Neoscytalidium (Scytalidium) dimidiatum, N novaehollandiae, N hyalinum, Botryosphaeria dothidea, Macrophomina phaseolina, Aureobasidium melanogenum, A namibiae, Arnium arizonense | BT | KF020895 | 54 | 105/90 | 1.02E+08 | 110 | 100% |
Scopulariopsis brevicaulis | Scopulariopsis brevicaulis, S alboflavescens, S cordiae, S flava, S insectivora, S stercoraria | ITS Ribosomal | LN850791.1 | 60 | 120/107 | 1.02E+08 | 136 | 100% |
Trichophyton interdigitale / mentagrophytes | Trichophyton interdigitale, T mentagrophytes, T benhamiae, T equinum, T indotineae, T quinckeanum, T tonsurans, T schoenleinii, T simii, T verrucosum, T violaceum, Arthroderma benhamiae, A simi, A vanbreuseghemii | ITS Ribosomal | NR_144900.1 | 65 | 130/108 | 1.02E+08 | 4066 | 100% |
Trichophyton rubrum / soudanense | Trichophyton rubrum, T soudanense, T gourvilii, T kuryangei, T raubitschekii, T violaceum, T yaoundei | ITS Ribosomal | NR_131330.1 | 64 | 128/104 | 1.02E+08 | 1873 | 100% |
- The Amplicon is the amplified piece of DNA which contains the Fwd, Rev and Probe sequence. Sequence between the Fwd, Rev and Probe are also included in Amplicon.
- The BLAST Score describes how similar the Amplicon sequence is to aligned sequences in the non-redundant GenBank Database. The range shown as High/Low reflects the highest and lowest scores for a pathogen that would generate a positive response using this assay.
- The number of sequences reviewed reflect the total number sequences in the non-redundant GenBank database on the date of the analysis. This number will change as new sequences are added to the GenBank database.
- Matches reflect the total number of pathogens identified in the BLAST search that would generate a positive ressponse. Statistically these are the number of True Positives (the diagnostic test would indicates the presence of the correct disease) = TP
- False positive (FP) – Test incorrectly detects the presence of pathogen in a sample that does not contain the pathogen. Shown as an incorrect pathogen in the BLAST analysis with a Score within the High/Low range listed for the assay.
True negative (TN) – Test correctly identifies absence of pathogen. Calculated from BLAST data as the (total number of sequences reviewed) – (total number of pathogens correctly detected or TP).
Specificity = TN / TN + FP
https://lexjansen.com/nesug/nesug10/hl/hl07.pdf
* If you have inquiries about specific strains, please contact DTPM support.