Antibiotic Resistance Molecular Testing Kit

ANTIBIOTIC RESISTANCE KIT

The Antibiotic Resistance Kit is a comprehensive kit detecting, by molecular technique, 32 possible antibiotic resistance genes.

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Now your lab can provide healthcare providers with the quickest and most accurate results possible to allow them to determine an accurate diagnosis:

  • Expedited results
  • Multiple pathogens available
  • Unmatched accuracy of PCR
  • Simple collection
  • Direct access to experienced scientific staff
  • Personal customer service experience

For Research Use Only. Not for use In diagnostic procedures.

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PATHOGENS DETECTED IN THE DTPM ANTIBIOTIC RESISTANCE KIT:

Pathogen Strain(s) Identified* Target Gene GenBank Accession Number Amplicon1 (base pairs) BLAST Score2 Range (High/Low) Sequences3 Reviewed Matches4 (True Positives) Specificity5
CMY 1 blaCMY-1, 8, 8b, 9-11, 19, MOX 1-4, 8, 10, 11 blaCMY-1 NG_048768.1 67 131/111 1.02E+08 116 100%
CMY 2 CMY-2, 4-7, 12-18, 20, 22-25, 27, 30-35, 38, 39, 42-46, 49, 53-62, 64, 69, 71, 73, 77, 80, 86, 94, 95, 96, 99, 102, 106-108, 111, 119, 121, 124-126, 129, 130, 132-134, 138-141, 145-48, 153, 155, 156, 158, 160-167. IcAC2 blaCMY-2 AB672503.1 58 116/106 1.02E+08 1521 100%
CTXM 1 CTX-M-1, 3, 10, 12, 15, 22, 23, 28, blaFEC-1 blaCTX-M-1 NZ_AP024521.1 64 128/114 1.02E+08 5823 100%
CTXM 2 CTX-M-2, 4, 4L, 5, 6, 7, 20, blaToho-1 blaCTX-M-2 CP091665.1 57 114/107 1.02E+08 376 100%
CTXM 8 CTX-M-8, 40, 63, blaKLUG-1 blaCTX-M-8 AB543595.1 58 113/108 1.02E+08 84 100%
CTXM 9 CTX-M-9, 13, 14, 16, 17, 19, 21, 24, 27, blaTOHO-2, blaCTX-M-9 NG_049043.1 63 123/118 1.02E+08 2845 100%
CTXM 25 CTX-M-25, 26, 39, 41, 78, 89, 91, 94, 100, 152, 160, 185, 205, 217 blaCTX-M-25 NG_048974.1 59 118/101 1.02E+08 53 100%
ermB ermB ermB MG778119 66 132/122 1.02E+08 2690 100%
FOX blaFox-1-10, 12-16 blaFOX NG_049098 80 143/133 1.02E+08 63 100%
KPC KPC-2-8, 10-35, 142, 167 blaKPC CP078006.1 58 116/111 1.02E+08 2615 100%
MecA mecA mecA CP000255 81 162/128 1.02E+08 1596 100%
NDM NDM (1-24) blaNDM NG_049326.1 56 112/107 1.02E+08 3336 100%
OXA 1 blaOXA-1, 4 blaOXA-1 NC_005327.1 60 120/112 1.02E+08 2244 100%
OXA 2 blaOXA-2, 3, 21, 32, 46 blaOXA-2 NG_049496.1 58 113/106 1.02E+08 381 100%
OXA 48 blaOXA-48, 48b, 81, 162, 163, 165, 181, 199, 204, 232, 244, 247, 252, 370, 405, 416, 484, 514, 515 blaOXA-48 NZ_CP030135.1 58 116/96 1.02E+08 1466 100%
qnrA 1-9 qnrA 1-9 qnrA 1 NG_050462 82 146/127 1.02E+08 454 100%
qnrB 1 qnrB 1-3, 6, 13-18, 20, 23, 26, 29, 30, 41-43, 45, 48, 49, 52, 54, 57, 58, 64, 66, 75, 77, 80 qnrB 1 NG_050469 89 160/130 1.02E+08 1734 100%
qnrB 5 qnrB 5, 10, 19, 36, 40, 46, 47, 50, 56, 59, 61, 62, 67, 68, 70, 71, 72 qnrB 5 NG_050513 85 161/131 1.02E+08 1789 100%
qnrS qnrS 1, 3-5, 7-9 qnrS1 DQ485529.1 66 123/118 1.02E+08 2505 100%
SHV SHV-1, 2, 2a, 11, 12, 27, 28, 31, 33, 38, 41, 42, 61, 71, 77, 190, 201, 205, 206, 208, 209, 211-228 blaSHV CP043859.1 71 142/127 1.02E+08 5014 100%
Sul 1 sul1 sul1 NG_048102.1 54 108/101 1.02E+08 8691 100%
Sul 2 sul2 sul2 NG_048108.1 62 124/114 1.02E+08 5963 100%
Sul 3 sul3 sul3 NG_048120 67 134/113 1.02E+08 1068 100%
TEM TEM-1-7, 9, -14, 24, 52, 135, 235, 236, 237, 240 blaTEM NC_018106.1 94 182/170 1.02E+08 28448 100%
Van A vanA vanA CP014531.1 51 102/92 1.02E+08 536 100%
Van B vanB vanB AY958220.1 63 123/101 1.02E+08 159 100%
Van C 1 vanC1 vanC1 NG_048345.1 60 120 1.02E+08 50 100%
Van C 2-4 vanC2, vanC2/3, vanC3, vanC4 vanC2 NG_048408.1 107 190/185 1.02E+08 58 100%
VIM VIM-1-6, 8-12, 14-20, 23-66 blaVIM NG_050383.1 61 122/111 1.02E+08 1183 100%
VIM 7 VIM-7 blaVIM-7 NC_009739.1 56 112/102 1.02E+08 9 100%
VIM 13 VIM-13 blaVIM-13 NG_050340.1 53 106 1.02E+08 6 100%
Footnotes:

  1. The Amplicon is the amplified piece of DNA which contains the Fwd, Rev and Probe sequence. Sequence between the Fwd, Rev and Probe are also included in Amplicon.
  2. The BLAST Score describes how similar the Amplicon sequence is to aligned sequences in the non-redundant GenBank Database. The range shown as High/Low reflects the highest and lowest scores for a pathogen that would generate a positive response using this assay.
  3. The number of sequences reviewed reflect the total number sequences in the non-redundant GenBank database on the date of the analysis. This number will change as new sequences are added to the GenBank database.
  4. Matches reflect the total number of pathogens identified in the BLAST search that would generate a positive ressponse. Statistically these are the number of True Positives (the diagnostic test would indicates the presence of the correct disease) = TP
  5. False positive (FP) – Test incorrectly detects the presence of pathogen in a sample that does not contain the pathogen. Shown as an incorrect pathogen in the BLAST analysis with a Score within the High/Low range listed for the assay.
    True negative (TN) – Test correctly identifies absence of pathogen. Calculated from BLAST data as the (total number of sequences reviewed) – (total number of pathogens correctly detected or TP).

Specificity = TN / TN + FP
https://lexjansen.com/nesug/nesug10/hl/hl07.pdf

* If you have inquiries about specific strains, please contact DTPM support.

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